19. Rapidly detect as little as 0. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. The four reactions per sample were pooled, cleaned up with 1x AMPureXP beads, resuspended in 30 ul EB (10 mM Tris-HCl, pH 8. 0 ml) of the aqueous working solution of Quant-iT™ PicoGreen dsDNA Reagent was added to each cell sample, which was incubated for 10 min at 37 °C in the dark. The assay was prepared for 184 aliquots of DNA sample and five DNA standards, including one blank. The assay is linear over three orders of magnitude and has little sequence. n = 3. • Suitable for use in 96- and 384-well plate formats. Touch devices users use touch & swipe gestures. The method for this assay follows the instructions in the product information sheet for Quant-iT PicoGreen dsDNA Reagent and Kits from Molecular Probes, except that the assay volume is proportionately reduced from 2. Lambda DNA contained in the kit was used to create a six-point standard curve from 3. We use the Quant-iT™ PicoGreen® dsDNAkit for quantification of both extracted and amplified DNA. Using this data as a starting point, the optimal conditions (e. Compared with the aforementioned methods, the PicoGreen dye binding assay for dsDNA has greater sensitivity, specificity, and linear range. Quant-iT™ PicoGreen® dsDNA Assay Kit *2,000 assays* (includes PicoGreen® Reagent, 10X buffer, and standard λ DNA) Quant-iT™ RiboGreen® RNA Reagent *2,000 assays* 10 ng/µl to 100 ng/µl RNA (>500 bp; not specific for RNA over DNA) RNase protection assays Microarray samplesQuant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen ssDNA Reagent: ssDNA : 200 pg–2 µg : 500/525 : 2,000 : O11492 O7582: Quant-iT RiboGreen RNA Assay Kit Quant-iT. Picogreen assays are compatible with most microplate readers and fluorometers. In addition, the concentration of DNA obtained from the 1860 tomato samples was measured fluorometrically using Quant-iT™ PicoGreen™ dsDNA Assay Kit (hereafter PicoGreen, Thermo Scientific, Waltham, MA, USA) and a 96-wells plate reader VICTOR3 1420 (PerkinElmer, Waltham, MA, USA) equipped with an excitation filter F485 and. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. Automation of the Quant-iT™ PicoGreen® dsDNA Assay Kit on the Fluent. Description. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. 2 ng to 100 ng of DNA in the assay tube. Using this data as a starting point, the optimal conditions (e. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Three kits covering a wide range of dsDNA concentrations. Picogreen Assays sind mit den meisten Mikrotiterplatten-Lesegeräten und Fluorometern kompatibel. Antibodies Recombinant antibodies, monoclonal & polyclonal antibodies and primary & secondary antibodies. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. Easy to use UV absorbance alternative assay that can be. Each Helixyte™ assay kit is specially formulated with a target-selective DNA. (2012). 2 ng to 100 ng of DNA in the assay tube. Quant-iT™ PicoGreen ® dsDNA Reagent (10 vials, each containing 100 µL aliquots) 25 mL of 20X TE buffer; 1 mL of 100 µg/mL Lambda DNA (dsDNA standard) These jellies cheapest cialis uk and pills are can be ingested with or without food. The assay is highly selective for double- The assay is highly selective for double- stranded DNA over RNA (see “Assay selectivity” on page 1), and in the range of 0. The kits provide concentrated assay reagent, dilution buffer, and prediluted DNA standards. • Suitable for use in 96- and 384-well plate formats. 製品番号（カタログ番号）： P7581. Calibrator MPO. Unit Size. Thermo Fisher quant it picogreen dsdna quantitation assay kit. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. 86 / 100 stars. Rapidly detect as little as 0. 0 mL to 200 uL to fit a 96-well microplate format. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Q33130 Introduction The Quant-iT™ dsDNA Broad-Range Assay Kit makes DNA quantitation easy and. 50 ul PicoGreen Mix by swirling bottle. 3 Q32854 26. Unit Size. PicoGreen dsDNA quantitation reagents are. 5 ng/mkl – 100 mg/ml. Microplate reader setup for a Quant-iT 1X dsDNA HS Assay. 5ul of 12uM primer diluted in ClontechPicoGreen is highly linear (R 2 ≥ 0. It requires the most user preparation and calculation, but can be adapted for cuvettes or plates. Le réactif et les kits de quantification d’ADNdb PicoGreen sont parfaits pour les dosages PCR, les échantillons de biopuces, les dosages de lésions de l’ADN, les dosages d’activité enzymatique, la quantification d’ADN génomique, la mesure de l’ADNdb dans des mélanges complexes et la quantification d’ADN viral. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 1 mL kit. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. Libraries were quantified using the Quant-iT PicoGreen dsDNA Assay Kit (Life Technologies) and the Kapa Illumina GA with Revised Primers-SYBR Fast Universal kit (Kapa Biosystems). The DNA standard was Lambda DNA included in the Quant-iT PicoGreen dsDNA Assay Kit (ThermoFisher). Add 5uL of PicoGreen reagent for every 1mL of buffer. Quant It Picogreen Dsdna Staining Kit, supplied by Thermo Fisher, used in various techniques. For duplicate-well droplet. Samples ranging in concentration from 0 ng/mL to 1,000 ng/mL were measured in quadruplicate, and the results are shown from the same data plotted in two different ways (Tables 2 and 3). Begin typing to search, use arrow keys to navigate, Enter to select. Invitrogen™ Quant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents. Quant-iT PicoGreen dsDNA AssayキットおよびdsDNA試薬を使用すると、幅広いダイナミックレンジでdsDNA濃度測定を正確かつ精密に行うことができます。. Picogreen assays are compatible with most microplate readers and fluorometers. Working PicoGreen reagent was prepared by diluting the concentrated DMSO-PicoGreen stock solution, provided in the PicoGreen kit, 1:200 with TE according to the kit instructions. Thermo Fisher Scientific公司的Quant-iT PicoGreen dsDNA 检测试剂盒对DNA更特异，比传统光吸收方法的灵敏度高约 1000倍。 如产品手册所述，此实验在微孔板格式中采用单 一染料浓度的动态范围从250 pg/mL到1000 ng/mL。在这 里，我们证明了使用Molecular Devices公司SpectraMax®Quant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen ssDNA Reagent: ssDNA : 200 pg–2 µg : 500/525 : 2,000 : O11492 O7582: Quant-iT RiboGreen RNA Assay Kit Quant-iT. The lambda DNA standard, provided at 100 µg/mL in the Quant-iT ™ PicoGreen dsDNA Assay Kit, is diluted 50‑fold in TE to make the 2 µg/mL working solution. Quant-iT PicoGreen dsDNA Reagent. 2. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. were treated with standard-of-care chemotherapy in vitro followed by cfDNA quantification via a PicoGreen dsDNA assay. Quant-iT ™ PicoGreen ™ dsDNA Reagent 1 mL reagent 10 x 100 µL P7581 P11495 TE Buffer (20X), RNase-free 100 mL T11493. Contents and Storage Information. Also see our AccuClear® Ultra High Sensitivity dsDNA Quantation Kit, which is a great all. • Specific for dsDNA in the presence of RNA. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Quant-iT PicoGreen dsDNA Assay Kit 를 사용하면, ssDNA 와 RNA 그리고 free nucleotide 가 혼재되어 있는 시료에서도 dsDNA 만을 선택적으로 측정할 수 있으며, 25 pg /ml 의 매우 적은 양까지도 검출할 수 있습니다. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. Fluorescence was measured at 485/530 nm and plotted versus the mass of nucleic acid for the DNA alone,. You will need 100 ul diluted PicoGreen per well containing 100 ul sample. Free dye. 710 citations – Kit [P7589] Quant-iT™ PicoGreen™ dsDNA Assay Kit by Thermo Scientific. Quant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents. Qubit™ dsDNA Quantification Assay Kits. Picogreen assays are compatible with most microplate readers and fluorometers. RESULTS The OT-2 Constructs Highly Uniform LibrariesThermo Fisher double stranded dna quant it picogreen dsdna assay kit Double Stranded Dna Quant It Picogreen Dsdna Assay Kit, supplied by Thermo Fisher, used in various techniques. Picogreen assays are compatible with most microplate readers and fluorometers. Mit den Quant-iT Picogreen dsDNA-Assay-Kits und dsDNA-Reagenzien erzielen Sie präzise und präzise dsDNA-Konzentrationsmessungen über einen breiten dynamischen Bereich. The Rapidly detect as little as 0. このアッセイは、従来のUV吸光度法に比べて感度が何倍も高いため、様々な濃度範囲に対して最適化することが可能です。. Abstract. Prediluted standards provided with the Quant-iT assay were used to generate a standard curve. Table 2 summarizes results of the. Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile, distilled, DNase-free water. 99 to $1999. Rapidly detect as little as 0. Fluorometer. 2. 99 to $1999. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 3ng/mL，DNAThe fragment size of the amplicon was assessed using agarose gel electrophoresis and quantified by Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific). The PicoGreen dsDNA quantitation reagent and kits are ideal for PCR-based assays, microarray samples, DNA damage assays, enzyme activity assays, genomic DNA quantitation, measuring dsDNA in complex mixtures, and viral DNA quantitation. No. Microplate reader setup for a Quant-iT 1X dsDNA HS Assay. The dynamic range of this assay in microplate format, as stated in the product manual, is from 250 pg/mL to 1000 ng/mL with a single dye concentration. 2–400ng/µl (1µl sample in 200µl assay). For example, if the assay requires 50 wells, add 600 L of PicoGreen to 14. 1. Double stranded DNA quantification performed in down to 2 µl using the LVis Plate. • Suitable for use in 96- and 384-well plate formats. Catalog Number. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. dsDNA Assay Kit: Invitrogen Quant-iT PicoGreen dsDNA Assay Kits and dsDNA Reagents, spectrophotometer, qPCR Quantification Kit: Roche KAPA Library Quantification Kits — Complete Kit (Universal), and qPCR Plate Reader: LightCycler 480 Instrument II. Quant Ittm Picogreen Dsdna Assay Kit, supplied by Thermo Fisher, used in various techniques. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using the PicoGreen dsDNA quantitation assay and reagent. Results: DNA concentration values were measured by the “DNA Mamm. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. One microgram of DNA was sheared using an M220. Figure 2. Cloning, expression and proteins purification. Samples ranging in concentration from 0 ng/mL to 1,000 ng/mL were measured in quadruplicate, and the results are shown from the same data plotted in two different ways (Tables 2 and 3). 2023-11. Rapidly detect as little as 0. Technical note | Quant-iT and Qubit 1X dsDNA assays the Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. 2–100 ng of DNA. The AccuBlue® High Sensitivity assay is also available without any DNA standards ( 31008 ), for those that wish to use their own standard. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Regulations & Guidance; Manuals 0 0. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA,. Common contaminants such as salts, free nucleotides, solvents,Product Description. After a 5-minute. • Specific for dsDNA in the presence of RNA. Protocols All Picogreen reactions and measurements werethe Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. DNA was normalized to 2 concentrations, 100+/-20µg/mL and 20+/-5µg/mL. Quantify nucleic acids and proteins using four user-selectable fluorescent. Table 2 summarizes results of the. 1 mL kit. Total amount of 1X TE per assay will be 200 μl per well which includes the amount of TE used to dilute the PicoGreen reagent. Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. dsDNA standards were incubated in cuvettes and measured using a Varian Cary Eclipse. The Quant-iT™ dsDNA Broad-Range Assay Kit makes DNA quantitation easy and accurate. Description. All NGS libraries generated with both the Fragmentase and the FTP method contained similar amounts of ds DNA (800 ± 50 ng) and had similar mean insert sizes of the libraries in a range from 400 to 500 bp. Picogreen assays are compatible with most microplate readers and fluorometers. This protocol is a simplified and c. The assay is highly selective for double- The assay is highly selective for double- stranded DNA over RNA (see “Assay selectivity” on page 1), and in the range of 0. The PicoGreen dsDNA quantitation reagent and kits are ideal for PCR-based assays, microarray samples, DNA damage assays, enzyme activity assays, genomic DNA. Facebook. Average 86 stars, based on 1. Figure 2. Bioz Stars score: 99/100, based on 1 PubMed citations. Learn how to use the Quant-iTTM PicoGreenTM dsDNA Reagent and Kit to quantitate double-stranded DNA (dsDNA) in solution with a fluorometer or a microplate reader. 文件. 3. The PicoGreen assay has high specificity for dsDNA versus RNA or ssDNA, and is. Catalog Number. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. • Suitable for use in 96- and 384-well plate formats. (dsDNA) High Sensitivity (HS) assay, Quant-iT PicoGreen Assay, Bioanalyzer HS DNA assay, and NanoDrop Onec. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA. Catalog Number. stranded DNA (dsDNA). Quant-iT dsDNA Assay Kit, broad range Quant-iT 1X dsDNA high sensitivity; Target. Quant It Picogreen Dsdna Assay Kit Reagents, supplied by Thermo Fisher, used in various techniques. • Suitable for use in 96- and 384-well plate formats. We recommend using Quant-IT Picogreen as a high-sensitivity assay to measure DNA concentration in that range . The PicoGreen dsDNA quantitation reagent and kits are ideal for PCR-based assays, microarray samples, DNA damage assays, enzyme activity assays, genomic DNA. 2 - 100 ng/ml 480/520 NaCl, MgCl 2Quant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen ssDNA Reagent: ssDNA : 200 pg–2 µg : 500/525 : 2,000 : O11492 O7582: Quant-iT RiboGreen RNA Assay Kit Quant-iT. 2022. For example, 3 µL of the DNA standard mixed with 147 µL of TE is sufficient for the standard curve described in step 2. 5 – 1,000 ng/ mL DNA with a high dynamic range (Fig. 0 ml 200x in DMSO b. Note: Allow the Quant-iT ™ RiboGreen RNA Reagent to warm to room temperature before. Three kits covering a wide range of dsDNA concentrations. The Quant-iTTM PicoGreen® dsDNA Reagent is an ultra-sensitive fluorescent nucleic acid stain for quantitating double-stranded DNA (dsDNA) in molecular biology procedures. 双链DNA（dsDNA）定量检测试剂盒 【产品升级声明】： 我司（懋康生物）从22. 実験してみた – PicoGreenをプレートリーダーで使用してみた. is 1 pg/μL – 5 ng/μL so the sensitivity of this kit can be rated as Ultra High (UHS). The assay is linear over three orders of magnitude and has little sequence dependence. The PicoGreen is sticking to the sides of the cuvette or assay plate. no. It is specific for dsDNA in the presence of equimolar amounts of RNA. Table 2 summarizes results of the Quant-iT Prepare the first standard Combine 20uL of 100ug/mL lambda DNA from the Quant-IT PicoGreen dsDNA assay kit (Fisher P7589) with 980uL 1X TE to create a 2ug/mL standard. Briefly, 2 µL of sample was diluted in 498 µL TE buffer. The Quant-iT dsDNA High-Sensitivity Assay Kit and the Quant-iT dsDNA Broad-Range Assay Kit make DNA quantification easy and accurate. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. A Qubit dsDNA BR (broad range, 2 to 1000 ng) Assay Kit and Qubit dsDNA HS (high sensitivity, 0. The Quant-iT™ Picogreen® double stranded DNA reagent is added and mixed with both the samples and references through a controlled aspiration and dispense. For example, 3 µL of the DNA standard mixed with 147 µL of TE is sufficient for the standard curve described in step 2. 5), 200X PicoGreen. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. ZERO BIAS - scores, article reviews, protocol conditions and moreGot it. Catalog Number. 6 Add equivalent volume (100 μl) of diluted PicoGreen to every well (keeping plate in the dark as much as possible). PicoGreen reagent is diluted in dimethylsulfoxide (DMSO) which solidifies at refrigerator temperatures. Related applications: Nucleic Acid Quantitation. クイックオーダー. Selectively detect dsDNA from a wide variety of sources, and even in presence of. 10pg/µl vs. The AccuBlue® Broad Range RNA Quantitation Kit is highly selective for RNA over dsDNA. RCA products may be directly quantified using the Quant-iT® PicoGreen® dsDNA Assay Kit or with a Qubit® Fluorometer after dilution to the appropriate detection range. 2– 100 ng Quant-iT 1X dsDNA HS Assay Kit – Quant-iT PicoGreen Reagent 1–200 ng Quant-iT OliGreen ssDNA Reagent and Assay Kit. dsDNA assays. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. Quant-iT dsDNA BR buffer (Component B) 250 ml c. The superior sensitivity and performance of the Modulus™ Microplate Fluorometer allows for dsDNA detection as low as 250ng/mL. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. the automated setup to the manual setup of the Quant-iT PicoGreen dsDNA Assay Kit. Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile, distilled, DNase-free water. The Quant-iT dsDNA High-Sensitivity Assay Kit and the Quant-iT dsDNA Broad-Range Assay Kit make DNA quantification easy and accurate. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. HS Assay Kit or Quant-iT™ PicoGreen® dsDNA Assay Kit. The Quant-iT 1X dsDNA BR Assay Kit is accurate for initial DNA sample concentrations of 200 pg/µL to 4000 ng/µL (depending on sample volume. Qubit Flex NGS Starter Kit 1 instrument kit A45924 Microplate assays and instruments Quant-iT PicoGreen dsDNA Assay Kit 1,000 assays P11496 Quant-iT dsDNA Assay Kit, high sensitivity 1,000 assays Q33120 Quant-iT dsDNA Assay Kit, broad range 1,000 assays Q33130 Quant-iT RiboGreen RNA Assay Kit 1,000 assays R114909. PicoGreen®試薬アッセイは、蛍光を基にした迅速かつ簡単な手法により、dsDNAを正確に定量することができます。. Quantitation Range. Effect of the buffer dilution on the DNA measurement with the Quant-It PicoGreen dsDNA assay. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. Coleen - 06 Oct. Libraries were sequenced on the Illumina NovaSeq. Samples were excited at 480 nm and the fluorescence was measured at 520 nm using a TECAN Spark multimode reader. 2–100 ng, the fluorescence signal is linear with DNA. Triplicate 10 µL samples of DNA (), Escherichia coli rRNA or a 1:1 mixture of DNA and RNA were assayed with the Quant-iT dsDNA High-Sensitivity Assay Kit (). 2–100 ng and is selective for dsDNA, even in the presence of an equal mass of RNA. 适用于（设备）. The total amount of DNA expected is in the range of 3–100 ng, with concentrations as low as 0. Product Type. According to the obtained results, the assay presents a linear correlation in a wide range of 0. Picogreen assay kit (Invitrogen, P7589) a. The assay is highly selective for double- stranded DNA over RNA, and in the. Q33130) Metabolic (cholesterol) • Amplex Red Cholesterol Assay Kit (Cat. The Quant-iT™ PicoGreen® dsDNA Assay Kit makes for an easy, reliable, and accurate method to quantify dsDNA samples when used in conjunction with the Modulus™ Microplate Fluorometer from Turner BioSystems, Inc. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. Mit den Quant-iT Picogreen dsDNA-Assay-Kits und dsDNA-Reagenzien erzielen Sie präzise und präzise dsDNA-Konzentrationsmessungen über einen breiten dynamischen Bereich. Average size fragment was determined using a LabChip GX (PerkinElmer) instrument. 0 fluorometer (Selection Guide for the Qubit and Quant-iT Assay Kits. Prepare only the amount of 1:25 diluted PicoGreen required for that run. 86100 stars. Samples ranging in concentration from 0 ng/mL to 1,000 ng/mL were measured in quadruplicate, and the results are shown from the same data plotted in two different ways (Tables 2 and 3). Prepare the 1x TE buffer by diluting the 20x TE concentrate 1:20 by deionized water. Table 2 summarizes results of the. Development and characterization of a novel host cell DNA assay using ultra-sensitive fluorescent nucleic acid stain “PicoGreen. High tolerance for contaminants. Q33130). Quant-iT PicoGreen dsDNA Assay Kit. 使用 Quant-iT Picogreen dsDNA 定量试剂盒和 dsDNA 试剂在较宽的动态范围内实现精准的 dsDNA 浓度测定。. UV흡광 결과보다 민감도 우수. In addition to the Quant-iT™ dsDNA High-Sensitivity Assay Kit described here, we also offer the Quant-iT ™ dsDNA Broad-Range Assay Kit (Cat. ) Add PicoGreen to DNA samples Add 100 1x PicoGreen to each well of the DNA/standard plate without contaminating tips. In addition to the Quant-iT™ dsDNA High-Sensitivity Assay Kit described here, we also offer the Quant-iT ™ dsDNA Broad-Range Assay Kit (Cat. dsDNA • Quant-iT PicoGreen dsDNA Assay Kit (Cat. Sample fluorescence was. Both the Quant-iT High-Sensitivity dsDNA Assay Kit and the Quant-iT Broad-Range dsDNA Assay Kit provide concentrated assay reagent, dilution buffer, and pre-diluted DNA standards. Picogreen assays are compatible with most microplate readers and fluorometers. For example, if the amount of the linear dsDNA library available is 20 ng, the linear dsDNA library input should be 10 ng, and the linear dsDNA library concentration should be at least 0. Louis, MI, USA). Qubit® dsDNA BR Assay Kit. P7589. The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA. 50 pg to 2 µg. We quantified the extract with Quant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents (Thermos Fisher Scientific, Waltham, MA, USA) to evaluate the amount of residual DNA. A comparison of reagents for detecting and quantitating proteins in solution—Table 9. The fusion protein nsp7-nsp8 was generated by inserting a GSGSGS linker sequence. Sequencing was performed with a HiSeq Single-End Illumina platform (USA) [Citation 31]. pDNA encapsulation efficiency of LNP 10 and LNP 20 was measured by using a Quant-iT Pico-Green dsDNA assay kit (ThermoFisher Scientific, Waltham, MA, USA). Approximate fluorescence excitation/emission maxima: 510/527 nm (see Figure 1, page 2) Quant-iT ™ dsDNA Broad-Range Assay Kit Catalog no. Rapidly detect as little as 0. 0 fluorometer according to the manufacturer’s protocols; a sample volume of 1 μl was. Catalog Number. 作成者 LatB Staff 01. 2009;49(4):997–1002. 99. PicoGreen dsDNA quantitation reagents are. Description. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Quant-iT ™ 1X dsDNA Assay Kit, Broad-Range 4 ng−2 μg 1,000 reactions Q33267The supernatant containing the cytosolic extract was either immediately used or stored at − 80 °C until use. Simply dilute the reagent 1:200, load 200 µL into the wells of a microplate, add 1–20 µL sample, mix, then read the. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA,. The assay is highly selective for double-stranded DNA (dsDNA) over RNA (Figure 1, page 7) and is accurate for initial sample concentrations from 10 pg/µL to 100 ng/µL. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Picogreen Assays sind mit den meisten Mikrotiterplatten-Lesegeräten und Fluorometern kompatibel. Within two years of follow-up, the primary endpoint defined as nonfatal myocardial infarction, stroke or transient ischemic attack, cardiovascular death, and >80% target vessel restenosis occurred in 34 patients (43%). 04 corresponds to 2 μ g/mL dsDNA solution. The Quant-iT 1X dsDNA HS Assay Kit is accurate for initial sample concentrations ranging from 10 pg/µL to 100 ng/µL, providing a core detection range of 0. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. The Quant-iT RNA Reagent and Assay Kit makes RNA quantification easy and accurate. The Molecular Probes PicoGreen®. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. Picogreen assays are compatible with most microplate readers and fluorometers. 8 Measurement of growth factor content in. Reagent Search & Data Services Menu Reagent Search. Fluorescence signal measured for the DNA sodium salt standard from calf thymus using the Quant-iT™ PicoGreen® assay. The Quant-iT™ dsDNA BR assay can be adapted for use in cuvettes or 384-well microplates. • Suitable for use in 96- and 384-well plate formats. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. DNA standards ranging from 1000 ng/mL to 50 pg/mL were assayed using the Quant-iT PicoGreen dsDNA Assay and SpectraMax M5. Components of the Quant-iT™ Picogreen™ dsDNA Assay Kit, including the Picogreen reagent and 100 µg/mL λDNA standard stock (Thermo Fisher Scientific), were utilized. The high standard corresponds to the same concentration as the highest concentration dsDNA standard in the Quant-iT Assay Kits and can be diluted into the standard series. Picogreen assays are compatible with most microplate readers and fluorometers. PicoGreen dsDNA quantitation reagents are. High linearity and sensitivity over a broad DNA range. The fluorescence intensity as a function of the amount of dsDNA is measured at the optimal. PicoGreen dsDNA Assay Kit. This kit contains. Product Type. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. Replicates of four were added to a 96-well plate using 2 μL volumes for rows A through D (beige) and 10 μL volumes for rows E through H (blue). 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. 10. Before using PicoGreen dye to stain DNA, the concentrated Me 2 SO solution containing PicoGreen dye was diluted 200-fold using 1× TE buffer to prepare 2× PicoGreen working solution. 3. Catalog Number. PicoGreen is widely used in molecular biological procedures such as DNA fragment purification for subcloning, cDNA synthesis for library production as well as primer assays. 0 fluorometer and Quant-iT PicoGreen dsDNA. 1. Note: Allow the Quant-iT ™ RiboGreen RNA Reagent to warm to room temperature before. The kit contains: • PicoGreen dsDNA quantitation reagent, 1 ml, stock solution in DMSO • 20 x TE buffer, 25 ml, 200 mM製品番号（カタログ番号）： P11495. Accuracy of the Qubit dsDNA HS assay compared to the Quant-iT PicoGreen dsDNA assay plotted on two different types of curves. Rapidly detect as little as 0. 99. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using the PicoGreen dsDNA quantitation assay and reagent. g. The sample must yield a minimum of 55 ng of genomic DNA to ensure sufficient DNA for quality control (QC) and. 5 ng/ml ~ 1,000 ng/ml 까지 정비례. The Quant-iT™ dsDNA Broad-Range Assay Kit makes DNA quantitation easy and accurate. No. Quant It Picogreen Dsdna Assay Kit, supplied by Thermo Fisher, used in various techniques. Material Amount Concentration Storage Stability Quant-iT™ PicoGreen® dsDNA reagent (Component A) 1 mL in 1 vial (P7589) or in10 vials of 100 μL each (P11496) Solution in DMSO 2–6ºC Desiccate Protect from light • • • When stored as directed, product stable. (Center) The Quant-iT RiboGreen RNA Assay provides linear quantitation. After quantitation, the purified amplicons from each reaction mixture were mixed at equimolar ratios based on concentration and subjected to. • Specific for dsDNA in the presence of RNA. coli rRNA (Δ), or a 1:1 mixture of DNA and RNA (∎) were assayed in the Quant-iTTM dsDNA HS assay. Not for use in diagnostic procedures. For Research Use. ZERO BIAS - scores, article reviews, protocol conditions and morePicogreen assay with Qubit platform is considered to be the method of choice for DNA quantification. i) 110 sample x 150uL =16. Picogreen assays are compatible with most microplate readers and fluorometers. Unlike many other DNA quantitation methods, PicoGreen is minimally affected by the presence of lipid, protein, carbohydrates and other cellular. If you would like to use this kit with the Qubit® fluorometer, we have includedQuant-iT ™ PicoGreen ™ dsDNA Reagent 1 mL reagent 10 x 100 µL P7581 P11495 TE Buffer (20X), RNase-free 100 mL T11493. Pico Green dsDNA Assay 2 03/04/2019The assay was performed according to the initial MPO-DNA complex ELISA protocol, but blocking buffer, sample diluent and detection antibody diluent were replaced by an in-house blocking buffer. The kit contains. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 2–100 ng: 2000: O11492 O7582: Quant-iT OliGreen ssDNA Assay Kit and Reagent (Ex/Em = 498/518 nm) ssDNA: 1–200 ng: 2000: R11490 R11491: Quant-iT RiboGreen RNA Assay Kit and Reagent (Ex/Em = 500/525 nm) RNA: 20–1000 ng: 2000: Q32883:DNA concentration was quantified with Quant-iT PicoGreen dsDNA Assay kit (Invitrogen) on a Tecan plate reader. Using the Quant-iTTM PicoGreen® dsDNA reagent and the recommended assay protocol, researchers can quantitate dsDNA in the presence of equi molar concentrations of ssDNA and RNA with minimal effect on the quantitation results. 5mL. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Highly selective for dsDNA over ssDNA or RNA. Final libraries were quantified using a Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific) on a SpectraMax iD3 (Molecular Devices). The Quant-iT™ PicoGreen® DNA assay is the oldest assay and the most general-purpose. PicoGreen dsDNA quantitation reagent is a highly sensitive fluorescent nucleic acid stain which is used to quantitate double-stranded DNA. Materials: 1. 99 to $1999. The following protocol is intended for the quantification of double-stranded DNA using Quant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). Quantification of cfDNA was performed using a TECAN Safire plate reader (96-well format) in combination with the Quant-iT ™ PicoGreen ™ dsDNA Assay Kit (Molecular Probes, Invitrogen; cat #P11496, lot #1911829) according to the manufacturer’s instructions, with slight modifications. The Quant-iT PicoGreen dsDNA. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. Spectrofluorometry was performed based on guidelines from ThermoFisher Scientific Inc. This kit contains. Picogreen assays are compatible with most microplate readers and fluorometers. Quant-iT RNA BR (Broad Range) Assay Kit. Picogreen assays are compatible with most microplate readers and fluorometers. The QuantiFluor® dsDNA dye offers greater sensitivity over absorbance at 260nm (NanoDrop) low-concentration samples, and better or equal sensitivity performance when compared to Quant-IT™ dsDNA BR. DNA samples with a concentration determined by 260 nm absorbance higher than 100 ng/mL were diluted.